【摘要】 目的 :研究肺癌端粒酶活性及其临床应用价值。方法 :采用端粒重复序列扩增—微孔板杂交法 ,对 10 0例切除肺癌标本 ,分别取癌中央组织、癌旁 1cm、远癌组织 3块组织 ,进行端粒酶活性分析 ,同时送病理检查。结果 :端粒酶在肺癌组织中央部位的阳性检出率为 10 0 % ,癌旁 1cm为 84% ,远癌组织为 8% ,不同病理类型的肺癌端粒酶活性水平 ( A/mg蛋白 )分别为 :鳞癌 2 8例 0 .12 2 ,腺癌 6 8例 0 .12 5 ,小细胞癌 4例 0 .12 7,无显著性差异。结论 :端粒酶活性在切除肺癌标本中的测定 ,可望成为临床对其切除范围和预后进行判断的指标更多还原

【Abstract】 Objective: To study the activity of telomere enzyme in esophageal carcinoma. Methods: Activity analysis of telomere enzyme on sections of esophageal carcinoma in 150 cases, with 4 sections, one being from the center; one,1 cm by the carcinoma; one, 3 cm and one ,5-6cm using the method of TRAP-random millipore mating. Results: Positive detectable rate of telomere enzyme in the center part was 98.5%; 1 cm by it,72%; 3 cm, 42%; the positive detectable rate in the normal tissue 5-6 cm away from carcinoma was 4%. Conclusion: The determination of the telomere enzyme activity in esophageal carcinoma sections can be expected to be the judge for resection scope and the prognosis criterion.更多还原