【摘要】 使用反相高效液相色谱法， Ｚｏｒｂａｘ　 Ｏ Ｄ Ｓ Ｃ１８ ２５０×４．６ ｍ ｍ ５ μｍ 色谱柱，流动相为甲醇∶乙腈∶石油醚（３５∶５５∶１０），流速：１ ｍ Ｌ／ｍ ｉｎ，检测波长：４５０ ｎｍ ，建立 Ｒ Ｐ－ Ｈ Ｐ Ｌ Ｃ 分离测定 β－胡萝卜素顺、反异构体的方法．该方法在０．０１～１．０ ｍ ｇ／ｍ Ｌ 线性范围内峰响应值与其浓度呈良好的线性关系，ｒ＝ ０．９９９１．该方法可简便、准确分离检测 β－ 胡萝卜素顺、反异构体．更多还原

【Abstract】 A Reversed-phase high performance liquid chromatographic (RP-HPLC) method was used. A method for separation and determination cis and trans isomers of β -Carotene was developed by using a Zorbax ODS C 18 250×4.6 mm 5 μm column; mobile phase, methanol-acetonitrile-petroleum ether (35∶55∶10); flow rate, 1.0 mL/min; detection wavelength, 450 nm. The calibration curves was linear in the range of 0.01～1.0 mg/mL for all- trans - β -Carotene, r =0.9991. This method was found to be simple and accurate for determination cis and trans isomers of β -Carotene.更多还原