【摘要】 利用地高辛标记的０８５ ｋｂ 的鸡传染性贫血病毒 （ Ｃ Ａ Ｖ） 核酸探针， 对江苏某地区疑为 Ｃ Ａ Ｖ 的１５～３０日龄病鸡的肝 Ｄ Ｎ Ａ 样品进行斑点杂交。结果在被检的２０份样品中有６份为阳性，阳性率为３０％ 。对相同样品根据已知引物（特异性扩增出 Ｃ Ａ Ｖ 的０５８ ｋｂ Ｄ Ｎ Ａ） 进行 Ｐ Ｃ Ｒ 扩增， 所得结果与斑点杂交相同。对应的病鸡血清经间接免疫荧光试验 （ Ｉ Ｆ Ａ）， 有７份为 Ｃ Ａ Ｖ 抗体阳性， 与 Ｐ Ｃ Ｒ 扩增结果的符合率为８３％ 。初步结果表明， 江苏某地区存在 Ｃ Ａ Ｖ 感染。更多还原

【Abstract】 The chicken anemia virus(CAV)was detected by dot blot hybridization assay using the 0 85 kb PCR products labeled with Digoxigenin as probes.The special fragments(0 58 kb)of CAV DNA were amplified by the polymerase chain reaction(PCR).Using the two methods the tissue DNA samples of Jiangsu Province were detected and the same results showed that 6 out of 20 chicken flocks were positive(the positive rate was 30%).For the serums of the same samples,7 out of 20(35%)were positive by the indirect immunofluorescence assay(IFA).The result of IFA compared with the PCR/dot bloting showed 83% consistency.The preliminary investigation showed that the CAV infection is prevalent in the areas of Jiangsu Province.更多还原