【摘要】 选择香菇的野生株 （ Ｑ） 与栽培株苏香两种孢子单核体 （ Ａ１ Ｂ１， Ａ２ Ｂ２） 进行完全亲和双单杂交， 以拮抗试验辅以液体出菇试验初步鉴定双单杂交后代， 并以 Ｒ Ａ Ｐ Ｄ技术 （随机扩增的多态性 Ｄ Ｎ Ａ 技术） 对杂交后代进行检测。每个杂交组合只得到一个杂交后代， 说明在完全亲和双单杂交供体中存在先导核。运用原生质体单核化与交配型鉴定技术对杂交后代中来自供体的先导核进行了追踪研究。结果表明， 受体交配型不同时， 其先导核也不同。在 （ Ａ３ Ｂ３＋ Ａ４ Ｂ４） × Ａ１ Ｂ１双单杂交中先导核为 Ａ３ Ｂ３ 核， 在 （ Ａ３ Ｂ３＋ Ａ４ Ｂ４） × Ａ２ Ｂ２ 双单杂交中先导核为 Ａ４ Ｂ４ 核。更多还原

【Abstract】 Combinations between a wild type strain Q and two types of sporic monokaryons of a cultured strain Suxiang in Lentinula edodes (A1B1,A2B2)by the full compatiable di mon mating were carried out.The hybrids of di mon mating were preliminarily determined on the base of antagonic test,primordium formation on PDY medium and RAPD results.Only one hybrid was obtained from each mating combination and it showed that a pre entrance nucleus exists in the donor.The pre entrance nucleus in hybrids from the donor was investigated with the methods of monotikaryotization by protoplasting and determination of mating types.The results showed that the type of pre entrance nucleus was different and dependent on the mating type of the acceptor.The pre entrance nucleus was A3B3 in the (A3B3+A4B4)×A1B1,but A4B4 in the(A3B3+A4B4)×A2B2.更多还原