【摘要】 以１８年生杉木优树嫁接苗（一年生）为外植体进行离体培养，茎段的最高分化率为６９％，侧芽的最高分化率为４０％。以茎段为外植体，在改良的ＭＳ（氮元素减半）＋ＢＡＰ２．０ｍｇ／Ｌ＋ＩＢＡ１．０ｍｇ／Ｌ的培养基上，平均每个茎段可诱导不定芽３～４个。在继代培养中，以改良的ＧＤ培养基加高浓度的ＢＡＰ６．７ｍｇ／Ｌ，平均每个茎段可诱导不定芽１１～１３个，然后在ＭＳ培养基上生长；在继代培养中激素浓度一般由低到高，再由高到低的循环，可以明显提高繁殖率。在生根培养中，以ＭＳ培养基（氮元素减半）加ＩＢＡ１．５ｍｇ／Ｌ，根的诱导率在４４％。更多还原

【Abstract】 Tissue culture of Cunninghamia lanceolata has been conducted since 1970s, but rate of bud differentiation was low, and every stem could induce an average of 3～4 adventitous bud and used young stem was 1～5 years old. In order to study how to graft from the old to the young,and raise the rate of bud on high concentration of hormone in subculture. The plus tree of seed orchard (18 years old) was used to graft on 3/1997. And stem and bud from grafting (1 years old),were used to establish initial cultures on 10/1997 .The highest rate of stem was 69%,and the rate of bud was 40%. For the test of bud differentiation,every stem could induce an average of 3～4 adventitous bud on the improved MS (half decreasing N elemont ) with 2.0mg/L of BAP and 1.0mg/L of IBA.On the test of subculture,every stem could induce an average of 11～13 adventitous bud on the improved GD with the higher concentration of 6.7mg/L of BAP,and then, MS(no hormone) was establish. The reproductive rate, the concentration of hormone from low to high,and then from high to low on the subculture.On rooting culture,the rooting rate was 44% on (1/2N) MS with 1.5mg/L of IBA.更多还原