【摘要】 微卫星ＤＮＡ 是一种简单重复序列（Ｓｉｍｐｌｅ Ｓｅｑｕｅｎｃｅ Ｒｅｐｅａｔ，简称ＳＳＲ） ，其核心单位由２～４ 个核苷酸组成，两侧一般是保守序列。由于ＳＳＲ 具有共显性，多态性高，可进行ＰＣＲ 扩增分析，因此是一种很有价值的分子标记。微卫星在林木基因组中广泛存在，其核心单位主要是（ＡＧ）ｎ ，（ＡＣ）ｎ 。现已在许多林木中发现有微卫星，如：松树、杨树、桉树、苹果树。林木的微卫星标记可扩充现有的ＲＦＬＰ、ＲＡＰＤ、ＡＦＬＰ 遗传图谱，以及ＱＴＬ 分析，并应用于基因型鉴别，分子标记辅助选择育种。更多还原

【Abstract】 Microsatellites are simple,tandemly repeated di to penta nucleotide sequence motifs flanked by inique sequence.They are valuable as genetic markers because they are co dominant,detect high levels of allelic diversity and are easily and economically assayed by PCR.Experiments reveal that microsatellites are distributed throughout the forestry genome.The most abundant microsatellite motifs reported in forestry are (AG) n and (AC) n。The occurrence of unclear microsatellites has been reported in several forest tree species such as pine,poplar,eucalyptus,and apple.SSR can be expected to complement existing RFLP,RAPD,AFLP maps, and are useful for genotype identification.gene and QTL analysis,marker assisted selection in breeding and pedigree analysis.更多还原