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镉胁迫下水稻种子的萌芽生长及体内水解酶的活性变化

Effects of Cadmium (Cd) Stress on the Sprouting of Rice and Its Hydrolase Activities.

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【作者】 施农农; 陈志伟; 贾秀英;

【Author】 SHI Nong_nong et al. (Biology Department, Hangzhou Institute of Education, 310020),Agro_environmental Protection,1999;18(5):213~216

【机构】 杭州教育学院生物系!杭州310020;

【摘要】 利用室内培养方法研究了不同镉离子浓度胁迫下水稻种子的萌芽状况。结果表明,在镉胁迫下,水稻种子萌芽力受挫,生长势削弱,直至完全失活,与种子体内淀粉酶(Amy) ,酸性磷酸酯酶(Acp) 这二种主要水解酶活性受镉的剂量抑制效应有关。镉对水稻种子造成伤害的临界浓度为0 .05 m mol/L,抑制种子萌芽的浓度为0 .5 m mol/L,使种子完全失活的致死浓度为5 m m ol/L。对镉胁迫的反应Amy 较Acp 敏感,但二者活性大小均可作为鉴定水稻受镉害程度的酶学生理指标。

【Abstract】 Low sprouting rate, weak growing case and lethal phenomenon of rice result from the apparent decrease of the activities of amylase (Amy) and acid phos-phatase (Acp) in the bud of rice under cadmium stress . Suppose that low hydrolase activities may cause the lack of respiration material- glucose and the energy supply, then cause the transfer of metabolic products prevented, thus, numerous of new cells for shooting and rooting can not be constructed. The critical strength of cadmium harm to rice seed is 0.05mmol/L ,inhibition concentration to sprouting is 0.5mmol/L, lethal concentration (LC) is 5mmol/L, comparing to Acp, Amy shows more sensitive under Cd stress, but the variations of their activities can be both a physiological index in enzymology for detecting effects of cadmium on the sprouting of rice.

【关键词】 镉; 水稻种子; 淀粉酶; 酸性磷酸酯酶;
【Key words】 cadmium; rice sprouting; amylase; acid phosphatase;
  • 【文献出处】 农业环境保护 ,AGRO-ENVIRONMENTAL PROTECTION , 编辑部邮箱 ,1999年05期
  • 【分类号】X503
  • 【被引频次】104
  • 【下载频次】379
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