【摘要】 目的 了解无菌容器在反复开启使用中细菌污染情况。方法 将压力蒸汽灭菌后容器及在临床治疗室使用后24h内、24h、48h、48～72h的容器各30份采样作细菌培养。结果 容器灭菌后即作细菌培养及使用24h内无菌污染;使用24～48h、48～72h染菌率分别为76.67％、93.33％,平均菌落效分别为0.43cfu/cm~2、1.07cfu/cm~2,染菌量开启次数多的手术科比非手术科多,24h后有显著性差异(P<0.05)。结论 无菌容器盛装无菌物品在反复开启使用中存在污染,随着使用时间越长,染菌率越高,开启次数越多,染菌量越多。为控制院内感染,无菌物品最好采用单包装使用,有效期不能超过24h。更多还原

【Abstract】 ve To study the bacterial contamination of aseptic containers unsealed repeatedly. Methods From the containers just asepticized by high pressure stream and those used clinically for less than 24 hours, 24 to 48 hours, and 48 to 72 hours, we randomly took out 30 samples respectively to check their outcomes of bacteria cultivation. Results No bacteria could be found in the fresh aseptic containers or the ones used for less than 24 hours. But the others had all been contaminated by bacteria. In the containers used for 24 hours, 24 to 48 hours and 48 to 72 hours, the rate of contamination was 76.67% and 93.33% , and the amount of bacterial groups averaged 0.43cfu/cm2 and 1.07 cfu/cm2, respectively. Furthermore , the more frequently the containers were unsealed, the more the bacterial groups formed. The difference was significant ( P < 0.05). Conclusions The aseptic goods stored in an aseptic container can be conlaminaled if the container is unsealed repeatedly . The effective lime of asepsis is not more than 24 hours. The longer the time, the higher the rate of contamination. And the more frequently the containers are unsealed, the more the amount of bacteria are detected. So, in order to control the infection in hospital effectively, the aseptic goods had better be packed singly.更多还原