【摘要】 应用无筛选标记细胞株杂交瘤·杂交瘤细胞直接融合法制备了２株分泌抗慢性Ｂ淋巴细胞白血病（Ｂ－ＣＬＬ）Ｉｄｘ抗ＣＤ３双特异性抗体（ＢｓＡｂ）的四价体瘤细胞株。采用间接ＥＬＩＳＡ试验与间接免疫荧光试验证明了该ＢｓＡｂ能分别与Ｂ－ＣＬＬ患者血清及带ＣＤ３标记的细胞特异性结合，经细胞结合试验证明该ＢｓＡｂ同时具备与Ｉｄ及ＣＤ３两种抗原结合能力，经酶桥联法证明该ＢｓＡｂ亚类为ＩｇＧ１×ＩｇＧ２ａ，用ＭＴＴ法证实ＢｓＡｂ能诱导Ｔ细胞活化增殖更多还原

【Abstract】 quadroma cell lines secreting monoclonal anti idiotypic(Anti Id) and anti CD3 bispecific antibo dies (BsAb) to human B chronic lymphocytic leukemia (B CLL) were obtained by using direct B cell hybridization technique of hybridoma and hybridoma lack of screening markers. The specificity of BsAbs from culture supernatant or ascites was assayed by indirect ELISA, indirect IF and cell combination test. The results showed that BsAbs could specifically react with homologous serum IgM from patients with B CLL and cells carrying CD3 marker respectively, but also with Id and CD3 antigens simutaneously. The BsAbs belonged to IgG1×IgG2a subclass by sandwich ELISA. The biological characters of BsAbs were tested by MTT colorimmetry. The results showed that BsAbs could induce T cell proliferation and enhance cytotoxcity of LAK cells on target cells in vitro.更多还原