【摘要】 以大肠杆菌β－ｇａｌ基因为报告基因，优化了３种阳离子脂质体的转染条件，评估了其转染效率。用构建的含腺相关病毒反向末端重复序列（ＡＡＶ－ＩＴＲｓ）的质粒表达载体，经Ｄｏｓｐｅｒ介导将小鼠ＩＦＮγ基因导入ＭＭ４５Ｔ．Ｌｉ细胞，体外有效地表达ＩＦＮγ。瘤体内注射Ｄｏｓｐｅｒ－ｐＡＩ－ｍＩＦＮγ复合物可明显抑制肿瘤生长，荷瘤小鼠生存期延长。此简便、有效的非病毒转基因方法有望用于肿瘤基因治疗。更多还原

【Abstract】 In this study, we investigated the efficiency rates of liposome mediated gene transfection. Escherichia coli β galactosidase gene used as a reporter gene, three different cationic liposomes were compared for their transfection efficiency, and the transfection conditions were optimized. MM45T. Li transfected with Dosper plasmid complexes containing AAV ITRs and IFN γ cDNA in vitro, the efficient expression of INF γ gene was achieved. In BALB/c mice, the growth of tumors was significantly delayed and the survival time of the treated mice was markedly prolonged following intratumor injection of Dosper pAI mIFNγ complexes. This simplified and efficient nonviral gene transfer method might be used in cancer gene therapy.更多还原