【摘要】 为了探索内源性钠泵抑制因子在高血压发生中的作用，利用家兔免疫制备了抗哇巴因抗体及抗前海葱苷原Ａ 抗体，并对其特性进行了鉴定。利用该两种抗体和ＥＬＩＳＡ 法检测了高血压病人血浆及牛血清中哇巴因样及前海葱苷原Ａ 样钠泵抑制因子水平。检测结果发现原发性高血压病人血浆中存在哇巴因样物质水平（０．１３２±０．０２１ｍ ｏｌ／Ｌ）和前海葱苷原Ａ 样物质水平（０．１０２±ＤＦ８．８ｎｍ ｏｌ／Ｌ）；牛血清中哇巴因样物质的水平（１．６±０．２７ｎｍ ｏｌ／Ｌ）明显高于前海葱苷原Ａ 样物质水平（０．２２２±０．０８６ｎｍ ｏｌ／Ｌ）。证明内源性钠泵抑制因子哇巴因样及前海葱苷原Ａ 样物质在哺乳类动物血液里循环存在，是两种新的强心苷类固醇激素。更多还原

【Abstract】 To know the concentration of the isomer of ouabain substance and proscillaridin A like substance how correlate with hypertension, we raised antibodies against ouabain and proscillaridin A and tested its concentration in the plasma of patients with essential hypertension and bovine serum with an indirect enzyme linked immunosorbent assay. The results show that in human plasma exist ouabain like substance (0.132±0.021nmol/L)and proscillaridin A like substance (0.102±DF8.8nmol/L),and that in bovine serum the higher concentration of ouabain immunoreactivities (1.6±0.27nmol/L) than proscillaridin A immunoreactivities (0.222±0.086nmol/L). We concluded that both concentration of the ouabain immunoreactive and proscillaridin A immunoreactive materials circulating in blood correlates with the blood pressure in essential hypertension.更多还原