【摘要】 目的　探讨p53 、mdm2 基因蛋白改变在小细胞肺癌中的意义。方法　采用免疫组化法和聚合酶链反就—单链构象多态性分析 1 4例小细胞肺癌石蜡切片。结果　p53 阳性 9例 ,其中 2例为 ( +) ,7例为 ( ++～ +++) ,阳性率为 6 4 .3% ( 9 1 4) ,mdm2 阳性 4例 ,其中 1例为 ( +) ,3例为 ( ++～ +++) ,阳性率为 2 8.6 % ( 4 1 4)。p53基因第 5、6、7、8外显子 ,突变率分别为 2 1 .4% ( 3 1 4) ,1 4.3% ( 2 1 4) ,1 4.3% ( 2 1 4) ,7.1 % ( 1 1 4) ,总突变率为 5 7.1 %( 8 1 4)。结论　小细胞肺癌中存在较高的p53基因突变率和mdm2 基因蛋白的高表达率。更多还原

【Abstract】 Objective To discuss the significance of transform between p 53 and mdm 2 genes in small cell lung cancer (SCLC). Method p 53 and mdm 2 genes in 14 paraffin embedded tissue section were observed by immunohistochemical (IHC) stainging (LSAB method) and polymerase chain reaction and single strand conformation polymorsphism (PCR-SSCP) analysis. Results The positive rate of p 53 was 64.3%(9/14) of which 2 cases were +, 7 cases were ++～+++. The positive rate of mdm 2 was 28.6%(4/10) of which 1 case was + ,3 cases ++～+++.p 53 gene point mutation in exon 5,6,7 and 8 respectively: 21.4%(3/14);14.3%(2/13);14.3(2/14);7.1%(1/14).The total mutation rate of p 53 gene exists in SCLC.It is a negatively interretated between p 53 and mdm 2 (P<0.05).更多还原