【摘要】 目的：在癫痫动物模型上，探讨癫痫脑损伤性改变。方法：20只SD大鼠随机分为对照组和癫痫组，采用光、电镜技术和形态定量方法观察马桑内酯所致急性局灶型癫痫大鼠弓状核神经元损伤性改变。结果：两组间神经元胞体的面积分数和面数密度，神经元胞体和胞核的等效直径无显著差异（P＞0.05），癫痫组暗细胞内线粒体体积分数减少（P＜0．05）、粗面内质网则增加（P＜0．05），两者的面数密度无显著性改变（P＞0．05），溶酶体的体积分数及面数密度均增加P＜0．05）。亮细胞各细胞器及分泌颗粒未见明显改变（P＞0．05）。结论：以上结果提示癫痫大鼠弓状核神经元超微结构的改变，亮、暗细胞反应不同；癫痫引起神经内分泌功能的紊乱，可能与弓状核神经元损伤有关。更多还原

【Abstract】 Objective: To investigate the brain changes in epileptic animal models. Methods: 20 healthy adult male Spmp - Dawley (SD) rats were divided into control and epileptic groups randomly, the damage changes of areuate nucleus neurons in acute focal epileptic rats induced by Coriaria Lactone(CL) were observed with ligth and electron microscopic technique and morphometric method. Results: The areal fraction and numerical density on area of neuronal soma, and the equivalent diameter of neuronal soma and nucleus of arcuate nucleus showed no significant difference (P >0. 05) between control and epileptic group, the organelles and secretory guules of pale neurons in the areuate nucleus of the epileptic group had no significant change(P > 0. 05 ); in dark neurons eider; nevertheless, the areal fraction of mitochondria significantly decreased (P < 0. 05), while the areal fraction of the rough endoplasmic reticulmp sighcanily incnaed (P < 0. 05), the ntunerical density on area of the ndtochondria and endOPlasdric reticulums had no significant change(P > 0.05), the areal fraction and numerical density on area of the lysosome were beth significantly increased (P < 0.05). Conclusion: The above results showed that were the morphometric changs of the dark and pale neurons in the epileptic rat arcuate nucleus were differente, and the neuroendocrine disordels caused by epilepsy may be related to the areuate nucleus neurons damage.更多还原