【摘要】 目的　探讨血管紧张素转换酶（ＡＣＥ）基因的插入／ 缺失（ｉｎｓｅｒｔｉｏｎ／ｄｅｌｅｔｉｏｎ，Ｉ／Ｄ） 多态性与心肌梗死发病的相关性。方法　应用聚合酶链反应（ＰＣＲ） 扩增技术检测６３ 例心肌梗死患者及１１２ 例健康对照者的ＡＣＥ基因Ｉ／Ｄ多态性。结果　位于ＡＣＥ基因第１６ 内含子的Ｉ／Ｄ多态性经ＰＣＲ技术扩增后可分为三种基因型：纯合子缺失型（ＤＤ） ，纯合子插入型（ Ⅱ），杂合子插入／ 缺失型（ＩＤ）。６３ 例心肌梗死患者ＤＤ型２６ 例（４１％ ），ＩＤ型２４ 例（３８％ ），Ⅱ型１３ 例（２１％） ，Ｄ等位基因频率为６０％ 。１１２ 例对照者则分别为２５ 例（２２％ ），５０ 例（４５ ％） ，３７ 例（３３％ ） 及４５％ 。ＤＤ型及Ｄ 等位基因在心肌梗死组中显著高于健康对照组，（ Ｐ 值分别为０－００８ １ 及０－００４ ９）。心肌梗死患者ＤＤ型与非ＤＤ型在年龄、总胆固醇、甘油三酯、Ａｐｏ－ Ａｌ、Ａｐｏ－ Ｂ、吸烟以及所伴发的糖尿病、高血压等方面的差别均无显著性意义，但在甘油三酯及吸烟两项，表现出ＤＤ 型的心梗患者较非ＤＤ 型者有减低的趋势。结论　ＡＣＥ基因的插入／ 缺失多态性与国人心肌梗死发病相关，Ｄ等位基因及ＤＤ基因型可能为心肌梗?更多还原

【Abstract】 Objective An insertion/deletion(I/ D) polymorphism ofthe angiotensin Ⅰ- converting en zyme (ACE) gene has been postulated to be associated with an increased risk of coronary artery disease (CAD) and myocardialinfarction (MI) in European population , particularly in individuals without classic risk factors. This study is designed to probe into the relationship in Chinese population . Methods Polymerize chain reaction (PCR) was usedto determinethe genotypesforan inser tion/deletion polymorphism in intron 16 ofthe ACEgene in 63 cases of myocardialinfarction and 112 healthy sub jects as controls.Results The insertion/deletion poly morphism inintron 16 ofthe ACEgene was determined by PCRand categorized into three genotypes:two deletion alleles (genotype DD) , heterozygous alleles (genotype ID) ,andtwoinsertion alleles (genotype Ⅱ) .Theresults showed thatthegenotype pattern of DD,ID,Ⅱandthe D allele were 41 % ,38 % ,21 % ,60 % in 63 cases of MI and 22 % ,45 % ,33 % ,45 % in 112 control subjects. The DDgenotype and Dallele were more common in MI patients compared with control subjects (26/63 versus 25/112 , P= 0-008 1 and 60 % versus45 % , P= 0-004 9 ,respectively) .In the MIgroup ,although there were no significant deference in classic risk factors,such as smoking,triglyceride,cholesterol, Apo - A1 、Apo - B, etc, among each genotype ,there had a downtrend in smoking and triglyceride in genotype DDwhen compared with other two genotypes (IDand Ⅱ) . Conclusions The insertion/deletion polymorphism ofthe ACEgene is strongly associated with susceptibility to MI, and the genotype DDand Dallele seem to be a potentrisk factor of MIin a Chinese population.更多还原