【摘要】 从中国人血清中克隆出HBsAg adr亚型基因,采用pcDNA 3.1HisC真核细胞表达质粒进行基因重组。重组质粒pcDNA 3.1HisC-HBsAg分别用0.01、0.1、1、10和100μg腿部胫骨肌多点免疫BALB/C小鼠,4周时进行同样剂量加强免疫。初次免疫后4周和8周时分别检测HBsAg和HBsAb。结果表明,1μg以上剂量组加强免疫后,均可诱导小鼠产生特异性抗体。揭示HBV有发展DNA疫苗的可能性。更多还原

【Abstract】 HBsAg adr gene was cloned from serum of HBV carrier and reconstructed with pcDNA 3.1HisC which was designed for high-level expression and purification of recombinant proteins in Mammalian hosts. The BALB/C mice were injected with 0.01, 0.1, 1, 10 and 100μg recombinant pcDNA 3.1 HisC- HBsAg and enhanced immunization was given at 4 weeks interval. The results showed that protective level of specific humoral response was obtained by injecting more" than 1μg plasmid. It suggested the possibility of developing HBV DNA vaccine.更多还原