【摘要】 目的：研究胃癌ｃｅｒｂＢ２ 基因扩增。方法：使用荧光原位杂交技术（ＦＩＳＨ） 检测５５ 例胃癌。结果：胃癌肿瘤区ｃｅｒｂＢ２基因扩增阳性率为３６－４％ 。在胃癌移行区内阳性率为１０－９％ ，在正常粘膜区阳性率为３－６ ％ ，相互比较，三个区域之间差异有高度显著性（ Ｐ＜ ０－０１）。在肠型和弥漫型胃癌，ｃｅｒｂＢ２ 基因扩增阳性率为５１－６％ 和１６－７％ ，两者差异有显著性（ Ｐ＜０－０５）。ｃｅｒｂＢ２ 基因扩增阳性与淋巴结内胃癌转移有明显相关性（ Ｐ＜ ０－０５）。结论：ｃｅｒｂＢ２ 基因扩增与胃癌的发生，发展有明显关联，有助于判断胃癌预后。更多还原

【Abstract】 Purpose To investigate c erbB 2 gene amplification in gastric carcinoma. Methods Fluorescence in situ hybridization (FISH) was used to detect c erbB 2 gene amplification from 55 cases of gastric carcinoma tissues. Results The positivity rates of c erbB 2 gene amplification in cancer tissues was 36 4%. The positivity rate of c erbB 2 gene amplification in transitional areas of tumors were 10 9%, and those of c erbB 2 gene amplification in normal mucoepithelium regions of tumors were 3 6%. There were significantly difference among three areas ( P <0 01 or 0 001). The positivity rates of c erbB 2 gene amplification in intestinal type and diffuse type of gastric carcinoma were 51 6% and 16 7% respectively. There was significantly difference between two types ( P <0 05). The positivity rate of c erbB 2 gene amplification was related to lymph node metastasis of gastric carcinomas ( P <0 05). Conclusion c erbB 2 gene amplification is related to development and progression of gastric carcinoma, which might be useful in evaluating patients prognosis.更多还原