【摘要】 研究了以活化硅胶作吸附剂,固相反射吸光光度法测定钴.试验表明,在pH=4.0的乙酸钠-乙酸缓冲溶液中,0.05g活化硅胶对钴-PAN[1-（2-吡啶偶氮）-2-萘酚]的吸附达最大值,形成的绿色络合物的最大反射吸收波长为625nm,Co²⁺含量在0～6.0μg/25ml范围内,有良好的线性关系,检出限为8ng·ml^-1,相对标准偏差为4.97%.方法用于VB₁₂针剂中钴的测定,结果满意.更多还原

【Abstract】 It is reported in this paper that the active silica gel can be used as absorbent for Co²⁺-PAN complex in a NaOAc-HOAc buffer medium of pH 4 and the cobalt content in vitamin B₁2 can then be determined by the solid phase reflection spectrophotometry. The reflection absorption maxima is found to lie at the wavelength of 625nm. Linear relationship between the reflection absorption and concentration of cobalt is found to exist in the range 0⁶.0礸/25ml of cobalt ion. The limit of detection and the RSD are found to be 8ng ?ml^-1 and 4. 97% respectively- This method has been applied to the determination of cobalt in vitamin B₁2 injection.更多还原