【摘要】 目的：检测胃粘膜细胞表面是否存在与幽门螺杆菌（HelicobactorPylori，HP）特异性相结合的受体物质。方法：采用酶免疫亲和法检测胃、肝、肠组织细胞上的HP受体祥物质（HPReceptor－LikeSubstance，HPRLS），用大肠杆菌、空肠弯曲菌作受体的特异性试验以确定其特异性。结果：HPRLS仅在胃粘膜有较高的表达。与肝细胞、肠粘膜细胞相比差异具有显著性（P＜0.01）。特异性试验表明HPRLS仅能与HP结合，与大肠杆菌及空肠弯曲菌不能结合。结论：胃粘膜上皮细胞存在HP受体，肝细胞及肠粘膜细胞无此物质的表达。HPRLS只能与HP结合而不能与大肠杆菌、空肠弯曲菌结合，HP可能通过其表面的粘多糖与HPRLS相结合。更多还原

【Abstract】 Objective: To determine the expression of Helicobacter Pylori receptor - like Subtance (HPRLS) on the surfaceof gastric epithelial cells (GEC). Methods: The level of HPRLS was detected by an enzyme immunoaffinity assay on theGEC, hepatic cells, intestinal cells, respectively. The specificity of this method was assessed through blocking of EscherichiaColi and Campylobacter Jejuni. Results: Strong expression of HPRLS was found in GEC, but not in hepatic cells and intestinalcells (P <0. 01), moreover, this reaction could not be blocked in presence of Escherichia coli and Campylobacter Jejuni. Con-clusion: HPRLS existed only in GEC, meanwhile, our experiment suggested that this reaction occured in presence of mucosac-charide of GEC.更多还原