【摘要】 以药物敏感试验为标准，应用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）分析方法对６８例利福平耐药菌株（单耐利福平或多药耐药）和２０例利福平敏感菌株进行测定。结果８８例结核分枝杆菌均扩增出目标ＤＮＡ条带，１０例非结核分枝杆菌和８例非分枝杆菌未出现目标ＤＮＡ条带，所用引物扩增ｒｐｏＢ基因２１５ｂｐ片段为结核分枝杆菌复合群特异性的。６８例耐药菌株中６０例ＰＣＲ－ＳＳＣＰ条带与结核分枝杆菌标准菌株Ｈ３７Ｒｖ的条带有明显差别，２０例敏感菌株条带与Ｈ３７Ｒｖ条带一致。与药物敏感试验结果做对比，ＰＣＲ－ＳＳＣＰ发现结核分枝杆菌利福平耐药的敏感性为８８％，特异性为１００％。更多还原

【Abstract】 Eighty eight strains of clinical isolated Mycobacterium tuberculosis were analysed by PCR SSCP,with the drug susceptibility test as contral group.The objective DNA fragments were occured in eighty eight strains of clinical isolated Mycobacterium tuberculosis, but it not occured in ten strains of Mycobacterium non tuberculosis and in eight strains of non Mycobacterium. The primer used for amplify the 215bp of rpoB gene are specificity for Mycobacterium tuberculosis complex. Among the sixty eight rifampin resistant isolates,characterization of SSCP in sixty isolates of Mycobacterium tuberculosis are different from that in standard strain of M.tuberculosis. There are no difference between the twenty susceptible strains and the standard strain.In compare with the conventional susceptibility testing methods,the sensitivity and specificity of PCR SSCP for detection of rifampin resistance in sixty eight strains of M.tuberculosis was 88% and 100%,respectively.Using specific primer to amplify the fragment of rpoB,it can not only confirm the pathogen of M.tuberculosis, but can easily and rapidly detect rifampin resistance of M.tuberculosis by the PCR SSCP technique.更多还原