【摘要】 分别在庚型肝炎病毒 （ＨＧＶ） 基因５′端非编码区 （５′ＵＴＲ）、非结构 （ＮＳ） ３ 区及非结构 （ＮＳ） ５ 区设计套式引物，建立逆转录套式聚合酶链反应（ＲＴｎｅｓｔｅｄ ＰＣＲ） 检测血清ＨＧＶ ＲＮＡ。在２９９份不同血清标本中ＨＧＶＲＮＡ 阳性者４１ 例，基于５′ＵＴＲ、ＮＳ３ 区及ＮＳ５ 区引物ＰＣＲ的阳性率分别为８７８％ 、８０５％ 和９７６％ 。本研究结果表明根据中国株ＨＧＶ基因ＮＳ５区部分序列设计引物建立的ＲＴｎｅｓｔｅｄ ＰＣＲ更灵敏、实用。可用于对ＨＧＶ 感染流行病学及临床的研究。更多还原

【Abstract】 A reverse transcription polymerase chain reaction assay with nested primers(RT nested PCR)deduced from either the untranslated region(5′ UTR),nonstructural region 3(NS3)or nonstructural region 5(NS5)of the hepatitis G Virus genome was established to detect serum HGV RNA.Serum HGV RNA was tested in 299 at risk individuals.Of 299 serum specimen,41(13 7%)were positive for HGV RNA,the positive rate of detection with primer deduced from 5′ UTR、NS3 and NS5 were 87 8%、80 5% and 97 6% respectively.The results of this study suggest that PCR assays to detect HGV RNA with primers deduced from the sequece of partial NS 5 gene of HGV in chinese patients was more sensitive and practical,and could be used to study the HGV infection in China.更多还原