【摘要】 为探讨γ－ 谷氨酰转肽酶（ＧＧＴ） 在肝癌发生过程中表达及其变化。以病理组织学和生物化学方法，将自身配对的肝癌、癌旁组织和远癌组织中总ＲＮＡ 纯化，对总ＧＧＴ比活性和不同分子形式ＧＧＴ的表达与改变进行了研究。结果显示，在２４ 例肝癌、癌旁和远癌组织中：①癌灶→癌旁→远癌组织中，总ＲＮＡ 浓度呈逐步升高趋势，组织间的差异显著；②总ＧＧＴ、可溶性和膜结合性ＧＧＴ比活性，癌组织较癌周和远癌组织明显升高（Ｐ＜ ０－０５） 。肝癌组织中ＧＧＴ 过度表达，可能与肝组织中ＧＧＴ 基因的异常改变有关。更多还原

【Abstract】 To explore the expression and changes of γ-glutamyl transpeptidase (GGT) during the development of hepatoma.The concentration of total RNA,total GGT specific activities and alterations of different molecular forms of GGT in the self controlled liver cancer,adjacent paracancerous and distal cancerous tissues were investigated by pathological and biochemical assays.Of 24 hepatoma tissues:①An increasing tendency of total RNA concentration was found from cancer to distal cancerous tissues,and there was a remarkably different between hepatoma tissues and adjacent paracancerous / or distal cancerous tissues;②The specific activities (U/g) of total GGT,soluble GGT and membrane bounded GGT were significantly higher (P<0 05) in hepatoma tissues than those in adjacent paracancerous / or distal cancerous tissues.Overexpression of GGT in hepatoma tissues is probably correlated with abnormal alterations of GGT genes in hepatic tissues.更多还原