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习惯性流产夫妇B淋巴母细胞株的建立及实验室研究

Establishment of lymphoblast cell line in a habitual abortion couple and its experimental study

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【作者】 胡玉红王秀岩张险峰张春斌

【Author】 Hu Yuhong; et al

【机构】 佳木斯大学医学院

【摘要】 我们采用EB病毒转化微量全血法建立一具高SCE的习惯性流产夫妇的B淋巴母细胞株,其自发SCE为14.12次/每细胞,13.36次/每细胞,微核率为25.2%,21.2%,诱发SCE为22.76次/每细胞,22.16次/每细胞,微核率为38.3%,37.2%,与正常淋巴母细胞相比差异显著(P<0.01),本方法为研究习惯性流产提供了实验模型.

【Abstract】 We established a lymphoblast cell line in a habitual abortion couple by micro-whole blood method. Both the cell lines were of high SCE rate. They had higher SCE rate and micro nuclei rate, with 14.12times per cell, and 13.28 times per cell,respectively for SCE rate and 25.2% and 21.2%, respectively for micro nuclei ratc, Aner treated wito mitomytin, they had even higher SCE (38.3、 37.2)and micro-nuclei rate(10.7%, 10.2%)than normalβ - lymphoblast cell line.

  • 【文献出处】 黑龙江医药科学 ,HEILONGJIANG MEDICINE AND PHARMACY , 编辑部邮箱 ,1999年02期
  • 【分类号】R714.2
  • 【下载频次】17
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