【摘要】 为研究黑皮病患者细胞的生物学特性，我们用微量全血EB病毒转化法建立了黑皮病患者的B淋巴母细胞株（JMSSHH），并进行了核型分析及细胞生物学性状鉴定．该细胞呈悬浮生长，细胞群体倍增时间为46．7小时／代．核型为46XX。自发的姐妹染色单体互换（SCE）值为14．10±0．90，微核率24．60‰；丝裂霉素C（MMC）诱发的SCE值为23．60±1．80，微核率为41．90％，均明显高于对照细胞系（P＜0．01）．这些结果提示，该患者的临床症状可能与体细胞的DNA损伤修复功能缺陷有关．更多还原

【Abstract】 We established a lymphoblast cell line named JMS SHH, by coculture EB virus and peripheral blood lymphocytes of a black-skin pathient. The cell grows in a suspending status, and the cell doubling time is 46.7 hours per passage. The natural SCE value is 14.10±0.90, and the micronucleus test is 24.60 ‰. After induced with mitomycin C (MMC), the SCE value is 23.60±1.80, and the micronucleus ratio is 41.90‰.Compareing with nomal cell, there are very significant different between two cell lines (P<0.01). All the results indicate that the pathient’s clinical symptom is associated with the deficiency of DNA repairing function.更多还原