【摘要】 目的：观察ＩＬ－１和地塞米松对体外培养的人下颌髁状突软骨细胞一氧化氮（ＮＯ）生成的影响。方法：用硝酸还原酶法测定细胞培养上清液中ＮＯ终末代谢产物ＮＯ２－／ＮＯ３－浓度的变化。结果：单独使用ＩＬ－１，软骨细胞培养液中ＮＯ２－／ＮＯ３－水平显著升高。给予地塞米松能降低细胞培养液中ＮＯ２－／ＮＯ３－的升高幅度，但仍高于空白对照组。结论：ＩＬ－１能诱导体外培养的人下颌骨髁状突软骨细胞ＮＯ的生成，而地塞米松对这种效应有抑制作用。更多还原

【Abstract】 objective: To investigate effects of IL-1 and dexamethasone on the nitric oxide (NO) syntheses in human cultured TMJ articular chondrocytes.Methods: NO accumulation in the cultured cell supernatant was measured with nitrate reductase method and the Griess’ reaction. Results: The significantly increased NO 2 -/NO 3 - (the stable and products of NO) levels of cell supernatant were found after IL-1 application. The high concentration of NO 2 -/NO 3 - stimulated by IL-1 can be reduced by addition of dexamaethasone. Conclusions: IL-1 induced high level NO production in human TMJ chondrocytes in vitro, but this activity can be inhibited partly by dexamethasone.更多还原