【摘要】 目的 :探讨绞股蓝皂甙 (gypenosides,GP)及平阳霉素 (PYM )对口腔鳞癌颈淋巴结转移癌GNM细胞的体外抑制作用及作用机制。方法 :采用MTT法及流式细胞仪进行分析。结果 :GP与PYM对GNM细胞均有抑制作用 ,抑制率与药物浓度呈正相关 ,二者联用 ,抑制率增强。GP主要作用于细胞的“S”期。结论 :GP与PYM对GNM细胞具有抑制作用 ,GP对细胞的损伤是通过干扰细胞周期实现的 ,GP与PYM联用抑制作用增强在于二者作用的细胞周期不同。提示用药时可根据二者各时相时间分开应用。GP具有临床应用价值。更多还原

【Abstract】 Objective: To investigate the suppressive effect and effective mechanism of gypenosides (GP) and PYM on neck lymph node metatatic GNM cell line derived from human oral squamous cell carcinoma in vitro. Methods:MTT assays and flow cytometry were used. Results:There was a synergetic effect when GP was combined with PYM. a decrease in number of cells was noticed in "S" stage when GP was used. Conclusions:There was a suppressive effect when GP and PYM were used on GNM cell, a synergetic effect was realized by different cycle stage of two drugs. The present study provided evidence for using drugs clinically.更多还原