【摘要】 目的：本研究对抑癌基因ｐ５３进行基因克隆和表达以获得ｐ５３基因产物。方法：采用基因克隆和重组技术将Ｐ５３ｃ ＤＮＡ编码区重组到融合蛋白表达载体 ｐＧＥＸ－２Ｔ中，用免疫斑点杂交技术检测 ｐ５３融合蛋白在大肠杆菌中的表达。结果：我们获得了ｐ３基因融合蛋白表达质粒ｐＧＥＸ－ｐ５３并在大肠杆菌中获得了表达。结论：ｐＧＥＸ－２Ｔ是一个有效的融合基因表达载体，适合于ｐ５３基因的表达。用ｐＧＥＸ－ｐ５３可以很方便地获得ｐ５３蛋白，对研究Ｐ５３基因在口腔癌发生中的作用具有应用价值。更多还原

【Abstract】 Objective: The purpose of this study is to clone and express human tumor suppressor gene p53. Methods: Using the gene cloning and recombinant techniques, we got encoding sequence from human tumor supperssor gene p53, inserted it into fused protein expression plasmid pGEX-2T and detected p53 gene expression by immunoblot staining. Results: We obtained p53 gene expression plasmid PGEX-p53 and expressed the p53 gene in Ecoli. strain. Conclusion: PGEX -2T is an efficient expression vector for human p53 gene expression. p53 protein can be easily gotten by expressing PGEX - p53 in Ecoli. strain, which may provide us 53 protein to study its role in cell cycle of oral carcinogenesis.更多还原