【摘要】 本实验用原位杂交技术检测几种肿瘤组织的染色体畸变．用３号、８号和１２号染色体着丝粒特异性ＤＮＡ探针，对实体瘤组织的细胞染色体进行检测，发现大多数细胞的细胞核内有两个原位杂交点，代表一对正常的染色体．有的细胞有三个原位杂交点，显示该细胞有染色体三体．整个实验过程简单、快速、准确、易重复．对分析肿瘤的细胞有一定的意义更多还原

【Abstract】 Hybridization in situ technique was employed to detect the chromosomal aberration in tumor tissues.By using DNA probe specific for centromere of chromosome 3,8 and 12,detections were made on the chromosome of some fresh tumor tissues.The result showed that within most of the nuclei there existed two signals of hybridization in situ which represented one pair of normal chromosome,while in other nuchlei three signals of hybridization in situ were found which indicated the presence of trisomy.The whole detection process aws fast and accurate in analysis and could easily be repeated,which was of great value in the analysis of tumor cells.更多还原