【摘要】 为了检测血清高密度脂蛋白各亚类，采用琼脂糖及梯度聚丙烯酰胺凝胶双向电泳、免疫印迹试验和斑点扫描分析建立了血清高密度脂蛋白亚类的检测方法。高密度脂蛋白各亚类含量重复性试验的板间变异系数为４ ．９ ％ ～１１ ．１ ％ ，正常人血清测得的高密度脂蛋白２ｂ 、２ａ、３ａ、３ｂ 、３ｃ 亚类含量分别为３０ ．８４ ％ ±６ ．９５ ％ 、２１ ．６０ ％ ±３ ．８８ ％ 、２０ ．２８ ％ ±３ ．６９ ％ 、１３ ．５４ ％ ±２ ．０３ ％ 及７ ．０５ ％ ±１ ．７５ ％ ，前β１ 高密度脂蛋白和前β２ 高密度脂蛋白含量分别为６ ．６７ ％ ±１ ．２２ ％ 及４ ．５２ ％ ±１ ．１２ ％ 。结果表明所建立的方法可直接以血清为样品，同时准确检测α及前β高密度脂蛋白各亚类的组成及含量。更多还原

【Abstract】 Aim An immunodetection method was developed to determine the contents of serum HDL subclasses. Methods High density lipoprotein (HDL) subclasses in human serum were separated by two-dimensional agarose-gradient polyacrylamide gel electrophoresis, the molecular size and mass of the HDL subclasses were determined by immunoblotting test and density scanning. Results The variation coefficients of the contents of the HDL subclasses are 4.9%～11.1%. The contents of HDL 2b , HDL 2a , HDL 3a , HDL 3b , HDL 3c in normalipidic serum are 30.80%±6.95%, 21.60%±3.88%, 20.28%±3.69%, 13.54%±2.03%, 7.05%±1.75%, respectively; and that of pre β1-HDL and pre β2-HDL are 6.67%±1.22%, 4.52%±1.12%, respectively. Conclusion The results suggested the method developed could be used to determine the contents of α-HDL and pre-β HDL subclasses in serum sample.更多还原