【摘要】 系统测定了天蚕 Ａｎｔｈｅｒａｅａ ｙａ ｍ ａ ｍ ａｉ 吐丝结茧至成虫期脂肪体、血淋巴和卵巢中卵黄蛋白和可溶性蛋白总含量的动态变化。结果表明， 脂肪体是卵黄原蛋白 （ Ｖｇ） 合成场所， Ｖｇ 合成始于吐丝结茧后第４ 天； 脂肪体、血淋巴中 Ｖｇ 滴度在吐丝结茧后第４ 天开始上升， 化蛹后第６ 天或第８ 天达高峰， 成虫羽化第１ 天则明显下降。卵巢对 Ｖｇ 摄取始于化蛹第１ 天， 此后随蛹日龄逐渐上升， 并渐趋平稳。同一卵巢管中卵黄蛋白 （ Ｖｔ） 含量自顶端至基端随卵室增大而逐渐升高， 不同日龄蛹中相应序号卵室的 Ｖｔ 含量以日龄大者为高； 卵室中 Ｖｔ 含量与卵室体积大小呈正线性关系。电镜观察表明， Ｖｇ 被卵母细胞摄入后以卵黄体形式存在， 不同发育阶段卵巢中卵母细胞内卵黄体大小不同， 以早期者为小； 同一卵巢管中不同卵母细胞内卵黄体以顶端为小， 基端明显增大，且卵黄体呈网状更多还原

【Abstract】 In the Japanese oak silkworm, Antheraea yamamai , the titre’s dynamics of vitellogenin(Vg) or vitellin(Vt) and total soluble proteins in the fat body, haemolymph and ovary from larval cocooning to moth emerging were assayed. Vg was synthesized in the fat body, and its synthesis started on the 4th day after cocooning. The Vg titer both in the fat body and haemolymph began to increase on the 4th day after cocooning, reached the highest level on the 6th or 8th day after pupation, and markedly decreased on the 1st day after emergence. Vg initated to be uptaken by the ovary on the 1st day after pupation, and then the titer of yolk protein in the ovary gradually increased with the increase of pupal day age and finally reached a plateau level. In the same ovariole, Vt content in an egg chamber gradually grew with the increase of egg chamber volume from the apex to terminal egg chamber (closest to the oviduct). In contrast to Vt titer in the egg chamber of the ovariole in the pupa with older day age, it was markedly lower in the corresponding egg chamber of the ovariole in the pupa with younger day age. There was a positive linear relationship between Vt content and egg chamber volume. The results acquired by electron microscopy indicated that Vg uptaken by oocyte was stored as yolk body. The size of yolk body in an oocyte changed with ovary development. At earlier stage of ovary development, its size was relatively smaller. In the same ovariole, yolk body’s size showed smaller in the apex oocyte, and showed larger with a net shape in the terminal oocyte(closest to the oviduct).更多还原