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螺旋藻中藻蓝素的提取研究
Study on the Extraction of Phycocyanobilin from Spirulina Platensis
【摘要】 从螺旋藻中提取藻胆蛋白,用甲醇解离其硫醚键得藻蓝素。其最佳解离条件是:温度为40℃,解离时间为24h,V(甲醇)∶m (藻胆蛋白) =200∶1 。提取率可达1-5% ,产物的UV,IR和文献相符。藻蓝素发色团在酸性溶液中呈比较扩展型,而在中性溶液中呈比较闭合的结构。
【Abstract】 The chromophore of phycobiliproteins,phycocyanobilin,was cleaved from the protein of Spirulina Platensis with methanol.The conditions affecting the cleavage of phycocyanobilin were investigated.The optimum conditions were as follows:cleavage temperature 40℃,cleavage time 24 hours and methanol/phycobiliproteins 200∶1(V/m).The extracting yield of the product reached 1 5%.Its UV and IR spectral data were compatible with those in the literature.The conformation variation of phycocyanobilin in different solutions was studied.
【关键词】 螺旋藻;
藻胆蛋白;
藻蓝素;
【Key words】 Spirulina Platensis; phycobiliproteins; phycocyanobilin Foundation item:Granted by university natural science foundation of Guangxi(Guijiao Gaoke[1995]408);
【Key words】 Spirulina Platensis; phycobiliproteins; phycocyanobilin Foundation item:Granted by university natural science foundation of Guangxi(Guijiao Gaoke[1995]408);
【基金】 广西高校自然科学基金
- 【文献出处】 精细化工 ,FINE CHEMICALS , 编辑部邮箱 ,1999年06期
- 【分类号】TQ264.3
- 【被引频次】11
- 【下载频次】432