节点文献

酵母金属硫蛋白对g12细胞抗γ射线损伤的作用

EFFECT OF SACCHAROMYCES CEREVISIAE BD101Cu--METALLOTHIONEIN ON RESISTINGIRRADIATION DAMAGE IN G12 CELLS

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 薛宏伟林慰慈安丹舒建芬刘素萍林稚兰

【Author】 XUE Hong--Wei; LIN Wei-Ci; AN Dan(College of Public Health, Beijing Medical Unversity, Beijing 100083)SHU Jian--Fen; LIU Su--Pin; LIN Zhi--Lan(College of Life Sciences, Peking university, Beijing 100871)

【机构】 北京医科大学公共卫生学院!北京100083北京大学生命科学院!北京100871

【摘要】 用胞质阻断法微核试验和单细胞凝胶电泳法检测酵母金属硫蛋白(BD101Cu-MT)对(60)Co-γ射线诱发的g12细胞微核形成和DNA链断裂的影响。结果表明10和50μg/mlBD101Cu-MT处理,对1或3Gyγ射线诱发的双核微核细胞率和1Gyγ射线诱发的彗星细胞频率及核DNA迁移距离的增加有明显的抑制作用.提示酵母金属硫蛋白对γ射线诱发g12细胞的遗传损伤有拮抗作用.

【Abstract】 The effects of Cu-methllothionein from Saccharomyces cerevisiae(BD101 Cu-MT) on micronucleus formation and DNA sbrand breaks in g12 cellsinduced by 60Co r ray were investigated using cytochalasin B--blocked method andsingle cell electrophoresis assay. The results have shown that the trentment of 10 and50ug /mL BD101 Cu--MT could decrease the ratio of micromucleated binucleated cellsinduced by 1 or 3 Gy r--ray and inhibit the increase in frequency of comet cells anddistance of migration of nucleur DNA induced by 1 Gy r--ray. R suggest that there isa antagonistic effect of BD101 Cu-MT on genetic damage in g12 cells induced by y--ray.

【基金】 国家自然科学基金!39737021
  • 【分类号】R37
  • 【下载频次】45
节点文献中: 

本文链接的文献网络图示:

本文的引文网络