【摘要】 用胞质阻断法微核试验和单细胞凝胶电泳法检测酵母金属硫蛋白（ＢＤ１０１Ｃｕ－ＭＴ）对(6０)Ｃｏ－γ射线诱发的ｇ１２细胞微核形成和ＤＮＡ链断裂的影响。结果表明１０和５０μｇ／ｍｌＢＤ１０１Ｃｕ－ＭＴ处理，对１或３Ｇｙγ射线诱发的双核微核细胞率和１Ｇｙγ射线诱发的彗星细胞频率及核ＤＮＡ迁移距离的增加有明显的抑制作用．提示酵母金属硫蛋白对γ射线诱发ｇ１２细胞的遗传损伤有拮抗作用．更多还原

【Abstract】 The effects of Cu-methllothionein from Saccharomyces cerevisiae(BD101 Cu-MT) on micronucleus formation and DNA sbrand breaks in g12 cellsinduced by 60Co r ray were investigated using cytochalasin B--blocked method andsingle cell electrophoresis assay. The results have shown that the trentment of 10 and50ug /mL BD101 Cu--MT could decrease the ratio of micromucleated binucleated cellsinduced by 1 or 3 Gy r--ray and inhibit the increase in frequency of comet cells anddistance of migration of nucleur DNA induced by 1 Gy r--ray. R suggest that there isa antagonistic effect of BD101 Cu-MT on genetic damage in g12 cells induced by y--ray.更多还原