【摘要】 猪肺炎支原体(MycoplasmahyopneumoniaeMph)是猪气喘病的主要病原,现遍布于世界各养猪国家。国内外科技工作者对该病的防治作了大量的研究报道[1]。尽管如此,有关Mhp的致病性及毒力因子仍知之不详[2]。中国在Mhp的弱毒研究方面取得了突破性进展[3,4],经多年应用表明该弱毒...更多还原

【Abstract】 Mycoplasma hyopneumoniae( Mhp) is the primary agent of Mycoplasma( or enzootic) pneumonia of swine( MPS) and one ofthemostimportantdirectorindirectcauses ofporcine respiratory infectious disease.Three Mycoplasma strains,Mhp1 68,an artificially attenuated strain,a field strain,isolated and identified from a pig with MPS and proved to be virulent and a modelstrain Jwere grown in KM2 .After4 8— 72 h incubation at3 7℃ ,cells were harvested through centrifugation,then washed and suspended in0 0 1 mol L PBSto1 1 0 0 oftheoriginalcul ture volume.Suspended Mycoplasmassolutions solubilized with sodium dodecylsulfate( SDS) ,and protein profiles of the Mycoplasmas were showed using SDS PAGE.The three Mhp strains from diverse origin produced similar protein profiles,suggesting thatvirulentand avirulent Mhp strains mightpossessa species specificprotein profiles. Full size chromosomal DNA of Mph strains1 68and Jwere prepared by LMP agarose embedmentlysis cellmem brane method,chromosomal DNA size were estimated as1 0 0 0 kb by pulsed field gelelectrophoresis( PFGE) from the mobilities of these full length molecules compared with DNA size references,Saccharomyces cerevisiae.PFGE results indicated thateither Mhp strain or strain Jhad only one chromosomal DNA band.更多还原