【摘要】 目的：研究曲拉通Ｘ－１００（Ｔｒｉｔｏｎ Ｘ－１００，ＴＴＸ）是否会改变脑内递质及合成酶免疫组织化学定位特征，及是否与其浓度有关。材料与方法：观察了高、低浓度ＴＴＸ预处理脑切片，对胆碱乙酰化酶、Ｐ物质和γ－氨基丁酸样免疫反应神经元胞体及终未，在树鼩内侧隔核、斜带核及海马，免疫组化定位的影响。并在树鼩和猫脑探索了缓冲的低浓度梯度酒精替代 ＴＴＸ的可能性。结果：在树鼩脑，当 ＴＴＸ为 ０． １％时，内侧隔核、斜带核三类神经元胞体染色变浅，数量减少，甚至消失，而海马及内侧隔核、斜带核的Ｐ物质同及γ－氨基丁酸阳性终末明显增加。当ＴＴＸ为０．０１％时，以上情况正好相反。以１０％～２５％～４０％～２５％～１０％酒精ＰＢＳ（ｐＨ７．４）替代ＴＴＸ，在树鼩和猫的海马及内侧隔核、斜带核能相当好地同时显示γ－氢基丁酸和Ｐ物质阳性神经元胞体和终末。结论：不同浓度的ＴＴＸ处理脑切片，可能对脑内众多神经活性物质的免疫组化定位特征产生不同影响。提高或降低 ＴＴＸ浓度，分别有助于显示神经元终末或胞体。上述梯度酒精替代ＴＴＸ，在免疫组化光、电镜研究中，有助于同时显示阳性神经元胞体和终末。更多还原

【Abstract】 This paper was to find out if Triton X-100(TTX) treatment of brain tissue would change the features of immunohistochemical localization of neuroactive agents, and if the chance was related with the concentration of TTX. Materials and Methods: Higher and lower concentrations of TTX were designed to pretreat the brain sections, and the effects on the immunohistochemistry of choline acetyltransferase (ChAT), substance P (SP), and γ-aminobutyric acid (GABA) were evaluated in the immunoreactive neuronal somata and terminal fibers in the nuclei of the medial septum and the diagonal band (MS-NDB) and hippocampal formation (HI) in the tree shrew. Meanwhile, the possibility of the substitute of a buffered-ethanolic sequence for TTX was investigated in the brains of the tree shrew and cat. Results: When brain see.tions of the tree shrew were pretreated with 0. 1% TTX, the above three kinds of positive neuronal somata became faint, decreased, and even disappeared, while SP-and GABA-like immunoreactive (-LI) terminal fibers evidently increased in the MS-NDB and HI. When brain sections were pretreated with 0. 01 % TTX, in contrast, all the positive neuronal somata and their proximal process were well labeled, though the positive terminals markedly decreased. When brain sections were pretreated with 10% - 25 % - 40% - 25 % - 10% ethanol in PBS pH7. 4 instead of TTX, both GABA- and SP-LI somata and terminals were finely labeled in the HI and MS-NDB in the tree shrew and cat. Conclusion: TTX treatment of brain tissues might cause changes in immunohistochemical localization for a variety of neurotransmitters and their synthesizing enzymes, with higher or lower concentrations of TTX enhancing the staining of immunoreactive terminals or somata respectively. The buffered-ethanolic sequence pretreatment might be very helpful for the immunohistochemical revealation of neuronal somata as well as terminals with the same or different chemical natures at the light and electron microscopic levels.更多还原