【摘要】 目的 :探讨小肠粘膜萎缩的发生机制。材料和方法 :采用原位末端标记和免疫组化的方法 ,对正常和萎缩小肠粘膜上皮细胞凋亡的发生、分布及增殖细胞核抗原 ( PCNA)的表达进行对比研究。结果 :凋亡细胞主要位于肠绒毛的顶部 ,萎缩小肠粘膜上皮细胞凋亡的发生率明显高于正常小肠 ( P<0 .0 5) ;而 PCNA阳性表达细胞主要位于肠腺隐窝区 ,萎缩小肠粘膜上皮细胞 PCNA的阳性计数较正常小肠显著降低 ( P<0 .0 5)。结论 :肠绒毛顶部凋亡细胞数的增多和肠腺隐窝区细胞增殖能力的降低共同参与了小肠粘膜萎缩的发生更多还原

【Abstract】 Objective: In order to elucidate the mechanism of intestinal mucosal atrophy. Method: A contrasting study on the occurrence and distribution of apoptosis and the expression of proliferating cell nuclear antigen was made between the normal and the atrophic intestinal mucosal epithelia by using the TUNEL(terminal deoxynudeotidy L transfere mediated dUTP biotin nick end ladeling) method and the immunohistochemical staining. Results: The apoptotic cells were located mainly in the intestinal villous tops; the rate of apoptosis in the atrophic intestinal mucosal epithelia was significantrly greater(P<0.05) than that in the normal intestine, but PCNA immunopositive cells were located mainly in the intestinal crypt; the expression of PCNA was significantly reduced(P<0.05) in the atrophic intestine as compared with that in the normal intestine. Conclusion:It demonstrated that the occurrence of the intestinal mucosal atrophy was the result of the increasing apoptosis in the intestinal villous tops and the decreasing of proliferation in the intestinal crypt.更多还原