【摘要】 目的 :研究心衰时心肌细胞及亚细胞结构的形态学改变。材料和方法 :制作鼠升主动脉缩窄模型、制备鼠心衰时的游离心肌细胞。用细胞骨架抽提法制备细胞骨架扫描电镜标本。结果 :升主动脉缩窄心衰鼠心肌细胞骨架中微管增加、肌原纤维减少、线粒体固定装置破坏、线粒体发生移位、空泡样改变、T管开口明显增大。结论 :慢性心衰时心肌细胞形态及亚细胞结构均已发生明显的形态学改变更多还原

【Abstract】 Objective:To study the morphological changes of cardiac myocytes and the subcellular structure of overload hart failure. Material and Methods: The overloaded heart failure models of Wistar rat with ascending aorta banding were made and to prepare the isolated cardiac myocytes and the samples of SEM of cytoskeleton with Triton x 100 were prepared. Results: Cytoskeleton changed in rat heart when heart failure occurred. The increasing of microtubules, decreasing of myofilament, disruption of fixed apparatus of mitochondria, dislocation, vacuolation of mitochondria and the enlargement of T tube were observed. Conclusion: The morphology and subcellular structure of cardiac myocytes changed when heart failure occurred.更多还原