【摘要】 目的　研究妊娠早期小鼠子宫内膜层粘连蛋白（ｌａｍ ｉｎｉｎ，ＬＮ）定位和定量变化及其对胚泡着床的作用。方法　应用间接免疫荧光法、免疫组织化学ＡＢＣ技术及图像分析法进行ＬＮ定位和定量测定；利用子宫内注射ＬＮ抗体的方法探讨ＬＮ对小鼠胚泡植入的作用。　结果　动情期及妊娠早期小鼠子宫内膜内源性ＬＮ 在上皮和腺基膜及血管内皮基膜均有表达，并随着植入的发生基膜的ＬＮ 表达减弱，而内膜上皮中ＬＮ免疫染色逐渐增强；外源性ＬＮ 对小鼠胚泡粘附与植入子宫内膜不产生明显影响，层粘连蛋白抗体明显抑制小鼠胚泡着床。　结论　动情期及妊娠早期小鼠子宫内膜的层粘连蛋白主要存在于基膜，胚泡植入期间内膜上皮细胞内ＬＮ 的含量增加；ＬＮ在促进小鼠胚泡粘附与植入子宫内膜过程中起重要作用。更多还原

【Abstract】 Objective\ To study the localization and quantitation of laminin(LN) in mouse endometrium during early pregnancy and its role on blastocyst implantation. Methods\ LN in mouse endometrium was shown and measured with indirect immunofluorescence techniques, immunohistochemical ABC method and image analysis system. The effect of LN on the implantation was studied by using LN antibody intra uterine injection.Results\ The expression of endogenous LN was found in the basement membrane of epithelium, glands and blood vessels in uterine endometrium of both estrus and early pregnancy. And its was faint that LN present in basement membrane as the implantation occurs, but the LN immunostaining in the epithelial cells of endometrium was stronger. The exogenous LN has no obvious influence on blastocyst attachment and implantation. LN antibody intra uterine injection showed significant repressing effect on mouse blastocyst implantation. Conclusions\ The LN mainly distributed in basement membrane of endometrium during estrus and early pregnancy in mouse.LN content in the epithelial cells was increased during the implantation. LN in mouse endometrium may play an important role in the process promoting blastocyst attachment and implantation.\;更多还原