【摘要】 目的　研究生长抑制ＤＮＡ损伤基因（ｇｒｏｗ ｔｈ ａｒｒｅｓｔ ａｎｄ ＤＮＡ ｄａｍ ａｇｅ， ｇａｄｄ）对卵巢癌细胞生长抑制和凋亡的作用。　方法　ＲＴ－ＰＣＲ鉴定卵巢癌ＳＫＯＶ３ 和３ＡＯ细胞株ｇａｄｄ１５３ 基因表达。通过目的基因的克隆、载体构建技术，将目的基因ｇａｄｄ１５３ 重组于ｐｌＸＳＮ－ｎｅｏ 逆转录病毒表达载体；构建的ｐＬＸＳＮ－ｇａｄｄ１５３载体采用脂质体（ｌｉｐｏｆｅｃｔｉｎ）（ＤＯＴＡＰ）的方法，分别转染ＳＫＯＶ３ 和３ＡＯ细胞株；经Ｇ４１８ 抗性筛选；ＲＴ－ＰＣＲ表达鉴定；足叶乙甙（ＶＰ１６）诱导，采用流式细胞仪分析（ＦＣＭ）的方法检测转染前后肿瘤细胞的生长抑制和凋亡。　结果　ＳＫＯＶ３－ｇａｄｄ１５３ 细胞生长抑制在Ｇ１／Ｇ０ 期，部分细胞进入凋亡的状态；３ＡＯ－ｇａｄｄ１５３ 细胞生长抑制，未引起凋亡。　结论　转染ｇａｄｄ１５３ 基因的肿瘤细胞系，诱导表达后可诱发肿瘤细胞生长抑制在Ｇ１／Ｇ０期，并有细胞进入凋亡状态。证实ｇａｄｄ１５３ 基因参与了细胞生长抑制和凋亡过程。更多还原

【Abstract】 Objective\ To study the effects of growth arrest and DNA damage(gadd) genes on the growth and apoptosis in ovarian cancer cell lines. Methods We constructed a retrovirus(pLXSN gadd153)which was transfected into SKOV3 and 3AO with lipofectin (DOTAP),respectively, then screened by anti G418 and identified the expression of gadd153 genes by RT PCR. Apoptosis was analyzed using FCM and DNA ladder. Results Growth inhibition and the apoptosis were observed in the SKOV3 cells transferred with gadd153 genes and also in the 3AO cells transferred with the same genes but there was only growth inhibition with no apoptosis occured. Conclusions The expression of gadd153 could make tumor cells arrested in G1/G0 and apoptosis could be developed in some other cell lines.更多还原