【摘要】 本实验选用３０只不同月龄健康雄性Ｗｉｓｔａｒ大鼠（２０月龄、１２月龄、３月龄各１０只），应用免疫细胞化学、计算机图像分析、形态计量法和电镜研究大鼠腺垂体在衰老过程中生长激素（ｇｒｏｗｔｈｈｏｒｍｏｎｅ，ＧＨ）细胞的形态学改变。结果表明：腺垂体实质细胞总数、ＧＨ细胞数随龄显著减少，ＧＨ细胞直径也随龄下降，以青年组到中年组减少最为明显（Ｐ＜０．０５）。应用ＩＰ－８计算机图像分析系统发现，ＧＨ细胞面密度随增龄显著减少（Ｐ＜０．０５）。老年大鼠腺垂体实质细胞排列紊乱，出现间质及血管周的纤维化和大量退化细胞，ＧＨ细胞呈固缩的表现，ＲＥＲ池扩张，核蛋白体脱落更多还原

【Abstract】 Thirty healthy male Wistar rats of various ages (3、12、20 months after birth) were used for this experiment. The morphological alterations of growth hormone (GH) cells of adenohypophysis in aging rats were studied through immunocytochemistry、morphometry、computer image analysis and electron microscope. The results show. There were significant age related declines in the adenohypophysial parenchymal cell number, GH cell number and diameter, which were most marked in the transition from youth to middle age (P<0.05). Marked age related alteration was observed in GH cell surface density through IP 8 computer image analysis system. The adenohypophysial parenchymal cells in aging rats arranged disorderly. Interstitial、perivascular fibrosis and a great many of degeneration cells was seen in aging rats. GH cells pycnosed. The RER cristern dilated to various extent and RER degranulated.更多还原