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广西四川金矿床区土壤样品中蜡状芽孢杆菌的分离和鉴定

Isolation and identification of Bacillus cereus from soil samples of gold deposit in Guangxi and Sichuan

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【作者】 汤显春陈绳亮罗勤熊克娟谢树成周修高

【Author】 Tang Xianchun; Chen Shenliang; Luo Qin; Xiong Kejian(Wuhan Institute of Virology, Academia Sinica, Wuhan 430071)Xie Shucheng; Zhou Xiugao(Wuhan College of Earthen Science, Chinese Geology University, Wuhan 430074)

【机构】 中国科学院武汉病毒研究所!武汉430071中国地质大学武汉地球科学学院!武汉430074

【摘要】 从广西金芽、四川阿西所属的三个金矿区采集了土壤样品55份,采用选择性培养基从10-2稀释度的平皿中分离到阳性菌739株,从中挑取11株,其分出率为1.5%,并对分离株进行了光镜和电镜形态学观察,其大小为1~1.4×2.5~3.5μm;芽孢椭圆形.中生,没有孢囊和伴孢晶体,并对11株分离株的生理生化特性进行了测定,结果表明,除C17分离株V、P反应阴性有待进一步分析外,其余10株都与标准株完全相同,因此,应归属芽孢杆菌属蜡状芽孢杆菌(Bacilluscereus).

【Abstract】 The isolation and identification of Bacillus cereus from soil samples ofgold deposit in Guangxi and Sichuan Provinces were reported. Fiftyfive soil sampleswere collected from 3 deposits. A total of 739 Positive colonies were isolated byEYA selective medium and 11 isolates were picked out from the colonies, the rateof isolation is 1. 5%. The morphology of cells and spores of these strains was inves-tigated under light and electron-microscope. The cells are Grampositive rods, 1. 0to 1. 4 pm by 2. 5 to 3. 5 pm, The spores are elliptical, central and without sporani-um and parasporal crystal. The physiological and biochemical characteristics of 11strains were examined. The results indicated that 10 of them should be identified asBacillus cereus

【基金】 地矿部生物成矿作用重点学科生长点基金;湖北省科委重点项目
  • 【文献出处】 华中师范大学学报(自然科学版) ,JOURNAL OF CENTRAL CHINA NORMAL UNIVERSITY(NATURAL SCIENCES) , 编辑部邮箱 ,1999年02期
  • 【分类号】S154.3
  • 【被引频次】9
  • 【下载频次】208
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