【摘要】 采用不同方法提取的嗜水气单胞菌脂多糖（ＬＰＳ）作为抗原，对鳜注射免疫后，通过测定受免鳜血清中凝集抗体效价、白细胞吞噬活性和进行强毒攻击的方法，比较了不同方法提取菌体ＬＰＳ的免疫原性。结果表明，Ｂｏｉｖｉｎ 法提取的菌体ＬＰＳ的免疫原性最强，Ｐｈｅｎｏｌｗａｔｅｒ 法提取的菌体ＬＰＳ的免疫原性次之，而ＥＤＴＡ 法提取的菌体ＬＰＳ的免疫原性较弱。更多还原

【Abstract】 Manderinfish( Siniperca chautsi ) was immunized by injection with lipopolysaccharide(LPS) extracted by different methods. The immunogenicity of differently prepared LPS was compared by examining the agglutination antibody and phagocytic activity of leucocytes and challenging test with live Aeromonas hydrophila . The immunogenicity of LPS extracted by Biovin and Phenol water methods was higher than those extracted by EDTA method. No significant difference was observed between the former two methods.\;更多还原