【摘要】 不同浓度的五种化疗药物（顺铂、阿霉素、长春新碱、平阳霉素和更生霉素）以及碱性成纤维细胞生长因子（b FGF）分别作用于卵巢癌COCl细胞系,荧光法动态测定肿瘤细胞内Ca²⁺浓度.结果:①在静息状态下肿瘤细胞浆内游离Ca²⁺浓度是85.6±9.7nmol/L;在bFGF作用下胞内游离Ca²⁺浓度增高,若细胞外液中有CaCl₂,则胞内Ca²⁺浓度增高.②在化疗药物作用下,肿瘤细胞内游离Ca²⁺浓度呈短暂快速下降,随后恢复到初始浓度乃至稍高于初始浓度,但各药各剂量对Ca²⁺的影响无明显规律性.更多还原

【Abstract】 Five chemotherapeutic drugs with different concentrations and basic fibroblast growth factor (bFGF) were used on the COC1 ovarian cancer cells. We measured the concentration of calcium in the cells with fluorescent way. Results :①The concentration of calcium in cancer cells was 85. 6±9.7 nmol/L when the cells were in a quiet state. The concentration of free calcium increased with the bFGF. If there was CaCl2 in outer liquid of cells,the concentration of calcium in the cells increased also.②The concentration of free calcium in the cells decreased shortly and rapidly and then it could resume to the original concentration or higher with the effect of chemotherapeutic drugs. But there was not obviously orderliness in the effect of the different doses.更多还原