【摘要】 目的探讨７ ｄ 和１４ ｄ 尾吊对小鼠脾脏淋巴细胞转化、ＩＬ２ 产生及ｃ－ｆｏｓ 基因表达。方法采用尾部悬吊小鼠模型模拟失重， ＭＴＴ法检测淋巴细胞转化， 生物活性法检测ＩＬ２ 产生， 点杂交检测ｃｆｏｓ 基因表达。结果尾吊１４ ｄ，脾淋巴细胞转化刺激指数显著降低，ＩＬ２ 产生明显减少；ｃｆｏｓ 表达在悬吊７ ｄ，１４ ｄ 均明显升高。结论尾吊可能对淋巴细胞调控基因产生影响。更多还原

【Abstract】 Objective To investigate the effect of simulated weightlessness on proliferation of spleen lymphocyte, production of interleukin 2 and expression of c fos proto oncogene in mice. Method Mice were tail suspended for 7 or 14 days to simulate the effect of weightlessness; proliferation of lymphocyte was measured by MTT method, production of IL 2 was determined by biological activity and expression of c fos proto oncogene was determinded by dot blotting. Result As compared with the control, the proliferation of spleen lymphocyte and the production of IL 2 were significantly decreased in 14 d suspended group, but the expression of c fos proto-oncogen was increased in both 7 d and 14d suspended groups. Conclusion This result suggested that simulated weightlessness could interfere with the genes regulating the lymphocyte.更多还原