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中国鲳属鱼类同工酶谱分析

ZYMOGRAM CHARACTERS OF GENUS Pampus (PISCES:STROMATEIDAE) FROM THE COAST OF CHINA

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【作者】 刘静; 尤锋; 李春生;

【Author】 LIU Jing; YOU Feng; LI Chun-sheng (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071 )

【机构】 中国科学院海洋研究所!青岛266071;

【摘要】 对中国近海鲳属鱼类的肌浆蛋白酶(Pm)、酯酶(EST)、乳酸脱氢酶(LDH)和超氧 化物歧化酶(SOD)等生化特征作了比较分析。结果表明,中国鲳属鱼类的Pm,EST和SOD 具有明显的种的特异性,而LDH则无种间差异。青岛、上海、厦门和珠海4个取样地点银鲳 的Pm和EST酶谱完全相同,同样,上述4个取样地点的翎鲳Pm和EST酶谱也完全相同, 从生化学的角度证明了我国沿海鲳属鱼类存在5个种,并且所有海区的银鲳和翎鲳各为同 一个种。

【Abstract】 Pomfret fishes of the genus Pampus are widely distributed in Chinese coastal waters and the Indowest Pacific. About 158 fish specimens of 5 Pampus species were collected from Qingdao, Shanghai, Xiamen, Zhanjiang, Beihai and Zhuhai during the Period from November 1996 to April 1998. Zymogram characters of myogens, EST isozymes, LDH isozymes and SOD isozymes were examined by polyacrylamid gel method. The zymo- gram patterns of myogens, EST and SOD isozymes of Chinese pomfret fishes showed remarkable differences. LDH isozymes had no species specificity. There were no obvious differences in Pm and EST zymogram patterns among those populations of P. argenteus and P. punctatissimus collected from Qingdao, Shanghai, Xiamen and Zhuhai respectively. These results were consistent with those obtained by morphological method.

【关键词】 鲳属鱼类; 电泳; 酶谱;
【Key words】 Pampus; Electrophoresis; Zmogram;
【基金】 中国科学院生物分类区系学科发展特别支持项目
  • 【分类号】Q178.53
  • 【被引频次】28
  • 【下载频次】205
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