【摘要】 对２２ 例人肝细胞癌及癌旁组织分别进行ＢｃＬ２、Ｂａｘ 的免疫组织化学研究和ＴＵＮＥＬ技术标记，探讨肝细胞癌中凋亡调控蛋白的表达与凋亡指数的关系。在肝细胞癌中ＢｃＬ２ 蛋白的表达率为２２－７３％ ，与癌旁组织相比无差异（ Ｐ＞０－０５）；Ｂａｘ 蛋白呈低表达，表达率为４５－４５％ ，与癌旁组织相比有显著差异（Ｐ＜０－０１）；肝癌组织中凋亡指数为９－５５％ ，低于癌旁组织１５－６８％ ，二者有显著差异（Ｐ＜ ０－０１）。肝细胞癌的发生与细胞凋亡受到抑制有关，Ｂａｘ 起到更关键的作用；ＢｃＬ２／Ｂａｘ 异二聚体能更准确地反映凋亡发生的程度；凋亡指数有可能成为判定细胞癌变的指标。更多还原

【Abstract】 To evaluate the relationship between apoptosis regulating gene and apoptosis index in human hepatocellular carcinoma,we observed the expression of BcL 2,Bax and apoptosis index using immunohistochemical staining and terminal deoxynuleotidyl transferase nick and labelling technique in 22 human hepatocellular carcinoma and paracancerous tissues.The rate of BcL 2 protein positive expression in human hepatocellular carcinoma was 22.73% and it was 18.18% in paracancerous tissues.No difference was seen as compared with each other ( P >0.05).The rate of Bax protein positive expression in hepatocellular carcinoma was 45.45% and it was 77.27% in paracancerous tissues.The apoptosis index in hepatocellular carcinoma was 9.55% and was lower than that in paracancerous tissues( P <0.01).There were relationship between the occurrence of hepatocellular carcinoma and the apotosis.BcL 2/Bax could reflect the degree of apoptosis accurately.Bax protein play an important role in the occurence of hepatocellular carcinoma.The apoptosis index would become an crucial value to evaluate malignancy change of cells.更多还原