【摘要】 为了用ＤＮＡ 序列分析仪快速、大批量地对大片段ＤＮＡ 进行序列分析研究，我们采用巢式定向渐进性截短缺失突变体构建方法，成功地对一株Ｃ型逆转录病毒基因的大片段ＤＮＡ进行了缺失突变体的构建。实验结果显示，该方法效果好，在大片段ＤＮＡ序列分析研究中具有推广应用价值。文内还对该方法的影响因素进行了讨论。更多还原

【Abstract】 This is a study aimed at the molecular mechanisms of β defensins gene expression and it’s gene transfer experiments for preventing mucosal infection. A rat rBD 1 cDNA was cloned. The total RNA was isolated from rat kidney. The cDNA fragment was amplified by RT PCR with specific primers. The purified RT PCR product was cloned in pGEM T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD 1 and mBD 1 demonstrated that rat β defensin rBD 1 gene was cloned successfully.更多还原