【摘要】 在ｐＨ６．０的乙酸－乙酸钠缓冲溶液体系中，有ＣＴＭＡＢ存在下，维生素Ｃ将铁（Ⅲ）－邻菲罗啉还原，还原产物与溴邻苯三酚红（ＢＰＲ）形成铁（Ⅱ）－邻菲罗啉－ＢＰＲ缔合物，其λｍａｘ＝５８０ｎｍ，ε＝１．０９×１０５Ｌ·ｍｏｌ－１·ｃｍ－１，维生素Ｃ含量在０～７０μｇ·２５ｍＬ－１范围内服从比耳定律。此法用于测定新鲜果蔬中的维生素Ｃ获得较满意的结果更多还原

【Abstract】 In HAcNaAc buffer solution (pH6.0) Fe()1,10phenanthroline is reduced by vitamin C to form Fe()1,10phenanthrolineBPR association compound in the presence of CTMAB. This compound has a maximum absorption at 580nm. Beer’s law is obeyed in the range of 070 g/25 mL. This molar absorptivity is 1.09105 Lmol-1cm-1. This method has been applied to the determination of vitamin C in several kinds of fruits and vegetables with satisfactory results.更多还原