【摘要】 目的:建立体外培养细胞加力装置,在细胞水平探讨机械力作用下大鼠髁突软骨细胞功能代谢的变化。方法:采用流式细胞术检测不同时段、不同力值的机械压力对体外培养的大鼠髁突软骨细胞增殖活性的影响。结果:在一定时间、0～2.03×10-4Pa(0～207g/cm2)力值范围内,随着机械压力的增大,受压的下颌髁突软骨细胞增殖活性增高,以持续压力作用6h最为明显;当持续压力作用于下颌髁突软骨细胞24h后,细胞增殖活性则出现下降。结论:在一定时间、一定力值范围内,随着机械压力的增大,受压的下颌髁突软骨细胞增殖活性有逐渐增高的趋势;当持续压力作用超过一定的范围,细胞增殖活性则出现下降趋势更多还原

【Abstract】 Objective:To study the proliferative changes of cultured rat mandibular condylar chondrocytes effected by mechanical compression.Methods: Using flow cytometry(FCM) to explore the changes of cellular DNA content and cell cycles of passaged condylar chondrocytes.Results: During certain force range (0~207 g/cm\+2), the proliferation of rat mandibular condylar chondrocytes rose, reaching the maximum after 6 hour application of force. The cellular proliferation went down when the chondrocytes were cultured under continuous pressure for 24 hours.Conclusion: The proliferation of rat mandibular condylar chondrocytes increase under certain force and certain period of time, but decreased if the applied pressure is beyond the magnitude.\;更多还原