【摘要】 研究了在膜分离式酶解反应器中单基质酶受叶轮剪切失活的现象。分别采用平板、刮刀、凹槽型式的叶轮在不同转速下进行实验，并在多级膜分离式酶解反应器中进行了验证。结果表明，不同的叶轮型式和叶轮转速对酶的剪切失活影响不同。将实验数据归纳为余存酶活与叶轮型式、叶端线速度、剪切暴露时间的曲线，应用它可估计酶在工作条件下的失活程度。更多还原

【Abstract】 The phenomenon that a single substrate enzyme deactivates in a enzymolysis reactor with membrance separation (ERMS) has been studied. The ERMS was composed of multiple reaction cells, in one of which was set with ultrafiltration membrane and an impeller. Enzymolysis reaction and membrane separation was performed simutameously when the unreacted substrate and the enzyme enter into every cell in turn. In order to elminate effects of concertration polarization and gel layer on ultrafiltration velocity and improve mixture degree of each reaction cell, various styles of impellers have been chosen to use. According to ERMS’s structure characters, the experiments have been run under conditions of various rotational velocity of three styles of impells such as plate, concavity and scraper. And the results have been proved by the industrial unit with 10 reaction cells. It has been concluded that impeller style and rotational velocity effect on enzyme deactivation. The experimental data have been sumarized curves of relic enzymolic activity relating to impeller style factor, linear velocity on the impeller edge shearing time. Those curves are practical to evaluate enzymalic deactivation degree for certain industrial performances.更多还原