【摘要】 目的：了解冬凌草甲素（Ｏｒｉｄ）及其它香茶菜属二萜类化合物香茶菜醛（Ａｍｅ）毛叶香茶菜醇（Ｉｓｏ）是否与冬凌草甲素同样具有抗氧化作用。方法：用分光光度法测定脂质过氧化物丙二醛（ＭＤＡ）含量及线粒体肿胀度。用荧光分光光度法测定线粒体膜流动性。结果：Ｏｒｉｄ，Ａｍｅ，Ｉｓｏ４０，８０，１６０μｍｏｌ／Ｌ抑制铁半胱氨酸（Ｆｅ２＋Ｃｙｓ）引起的肝线粒体ＭＤＡ形成，并呈剂量－依赖关系。Ｏｒｉｄ１６０μｍｏｌ／Ｌ抑制肝线粒体膜流动性下降。（Ｐ值分别为０２９７±０．０２２，Ｆｅ２＋Ｃｙｓ的Ｐ值为０４２３±０．０１４）；Ａｍｅ１６０μｍｏｌ／Ｌ还可抑制脂质过氧化引起的肝线粒体肿胀。结论：Ａｍｅ，Ｉｓｏ与Ｏｒｉｄ同样可能通过抑制脂质过氧化而产生抗氧化作用。更多还原

【Abstract】 Objective: To examine whether diterpenoid compounds have antioxidative action,the protective effects of diterpenoid compounds on rat liver mitochondria injury induced by free radicals were investigated Methods: Spectrometric methods were used to measure the content of MDA induced by free radical and the turbidity of mitochondria suspension.Fluorescence anisotropy assay was used to measure the mitochondria membrane fluidity.Results: Oridonin(Orid),Amethystonal(Ame) and Isodonoil(Iso) 40 ,80 and 160 μmol/L inhibited MDA formation of liver mitochondria in a concentration-dependent manner.Orid 160 μmol/L inhibited the increase of P value (0.297±0.022,vs 0.423±0.014 in ferrous-cysteine group).Ame 160 μmol/L prevented the decrease of the absorption induced by ferrous-ascorbic acid in liver mitochondria.Conclusion: Orid,Ame and Iso all have antioxidative effect through diminishing mitochondria lipid peroxidation induced by free radicals.更多还原