【摘要】 目的 :探讨食管癌发生发展过程中c myc基因表达的变化。方法 :用原位杂交方法 ,研究 2 1例食管鳞癌和相应癌旁粘膜中c myc基因的表达。结果 :食管正常粘膜、单纯增生、不典型增生Ⅰ、Ⅱ、Ⅲ级和原位癌的杂交阳性率分别为 12 5 % ,4 4 4 % ,5 2 9% ,5 3 8% ,5 8 3 %和 61 1% ,鳞癌Ⅰ、Ⅱ级分别为 5 0 0 %和 4 0 0 % ,其中单纯增生、不典型增生Ⅰ、Ⅱ、Ⅲ级、原位癌和鳞癌Ⅱ级的杂交阳性率较正常粘膜差异有显著性 (P <0 0 5 ) ;正常粘膜阳性杂交信号低于单纯增生至原位癌各病理组织类型 ,癌组织的杂交信号多明显高于相应癌旁粘膜。结论 :c myc基因在食管癌发生发展过程中的单纯增生阶段激活 ,c myc基因的高度表达可能作为食管癌变的原因之一而非癌变的结果更多还原

【Abstract】 Aim:To investigate the expression of c myc gene in the carcinogenesis of human esophageal (EC).Methods:21 cases of esophageal squamous cell carcinoma (SCC) and their corresponding mucosa were examined by using in situ hybridization (ISH).Results:The positive rates of c myc gene in normal esophageal epithelia,simple hyperplasia,grade Ⅰ,Ⅱ,Ⅲ of dysplasia,carcinoma in situ (CIS) and grade Ⅰ,Ⅱ of SCC were 12.5%,44.4%,52.9%,53.8%,58.3%,61.1%,50.0% and 40.0% respectively.The rates of normal epithelia were significantly lower than those of simple hyperplasia,grade Ⅰ,Ⅱ,Ⅲ of dysplasia,CIS and grade Ⅱ of SCC ( P <0.05).On the same slide,the hybridization signals in normal epithelia appeared weaker than those of other mucosa,while the signals in tumor were stronger than those of all mucosa.Conclusion:c myc gene may be activated at the stage of simple hyperplasia and its overexpression may be the cause,instead of the results,of human EC.更多还原